bk036 rac1 Search Results


cdc42 pull down activation assay biochem kit  (Cytoskeleton Inc)
95
Cytoskeleton Inc cdc42 pull down activation assay biochem kit
Myh9 assists Trio in the regulation of NCC migration via Rac1 and <t>Cdc42</t> GTPase activation. (A) Rac1 pull-down activation in the shCtrl, shTrio, and shMyh9 NCC groups, with quantification (n = 3). (B) Immunostaining of β-catenin (green) and Rac1 (red) in NCCs. Bar = 100 μm. (C) Immunostaining of β-catenin (green) and DAPI (blue) for nuclear β-catenin expression indicated by a white arrow in shTrio and shMyh9 NCCs. Bar = 100 μm. (D) Western blot of β-catenin in the nucleus and cytoplasm in the shTrio and shMyh9 NCC groups. (E) qRT-PCR of migratory NCC marker genes (Foxd3, Hnk-1, Snai2, Sox9, Twist-1, Pax7) expression (n = 5). (F) Dual-luciferase reporter assay of relative NCC marker genes (Foxd3, Snai2, Sox9, Pax7) influenced by β-catenin (n = 5). (G and H) Cdc42 pull-down activation assay in the shTrio and shMyh9 NCC groups, with quantification (n = 3). (I) Immunofluorescence staining of the Golgi body marker GM130 and F-actin for polarization assay. The nuclei were counterstained with DAPI. The following schematic shows a polarized and nonpolarized cell, as well as the angle of nucleus reorientation. Bar = 100 μm. The quantification is shown as the percentage of Golgi facing the wound and the angle of nucleus reorientation (n = 5). For (A), (E), (F), (H), and (I), data are represented as mean ± S.E.M. (two-tailed t test *p < 0.05, **p < 0.01).
Cdc42 Pull Down Activation Assay Biochem Kit, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bk036+rac1/pmc07977452-278-11-18?v=Cytoskeleton+Inc
Average 95 stars, based on 1 article reviews
cdc42 pull down activation assay biochem kit - by Bioz Stars, 2026-07
95/100 stars
  Buy from Supplier
kits bk036 and pak02  (Cytoskeleton Inc)
95
Cytoskeleton Inc kits bk036 and pak02
Myh9 assists Trio in the regulation of NCC migration via Rac1 and <t>Cdc42</t> GTPase activation. (A) Rac1 pull-down activation in the shCtrl, shTrio, and shMyh9 NCC groups, with quantification (n = 3). (B) Immunostaining of β-catenin (green) and Rac1 (red) in NCCs. Bar = 100 μm. (C) Immunostaining of β-catenin (green) and DAPI (blue) for nuclear β-catenin expression indicated by a white arrow in shTrio and shMyh9 NCCs. Bar = 100 μm. (D) Western blot of β-catenin in the nucleus and cytoplasm in the shTrio and shMyh9 NCC groups. (E) qRT-PCR of migratory NCC marker genes (Foxd3, Hnk-1, Snai2, Sox9, Twist-1, Pax7) expression (n = 5). (F) Dual-luciferase reporter assay of relative NCC marker genes (Foxd3, Snai2, Sox9, Pax7) influenced by β-catenin (n = 5). (G and H) Cdc42 pull-down activation assay in the shTrio and shMyh9 NCC groups, with quantification (n = 3). (I) Immunofluorescence staining of the Golgi body marker GM130 and F-actin for polarization assay. The nuclei were counterstained with DAPI. The following schematic shows a polarized and nonpolarized cell, as well as the angle of nucleus reorientation. Bar = 100 μm. The quantification is shown as the percentage of Golgi facing the wound and the angle of nucleus reorientation (n = 5). For (A), (E), (F), (H), and (I), data are represented as mean ± S.E.M. (two-tailed t test *p < 0.05, **p < 0.01).
Kits Bk036 And Pak02, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bk036+rac1/pm15277528-104-52-58?v=Cytoskeleton+Inc
Average 95 stars, based on 1 article reviews
kits bk036 and pak02 - by Bioz Stars, 2026-07
95/100 stars
  Buy from Supplier
activation assay biochem kit  (Cytoskeleton Inc)
96
Cytoskeleton Inc activation assay biochem kit
Myh9 assists Trio in the regulation of NCC migration via Rac1 and <t>Cdc42</t> GTPase activation. (A) Rac1 pull-down activation in the shCtrl, shTrio, and shMyh9 NCC groups, with quantification (n = 3). (B) Immunostaining of β-catenin (green) and Rac1 (red) in NCCs. Bar = 100 μm. (C) Immunostaining of β-catenin (green) and DAPI (blue) for nuclear β-catenin expression indicated by a white arrow in shTrio and shMyh9 NCCs. Bar = 100 μm. (D) Western blot of β-catenin in the nucleus and cytoplasm in the shTrio and shMyh9 NCC groups. (E) qRT-PCR of migratory NCC marker genes (Foxd3, Hnk-1, Snai2, Sox9, Twist-1, Pax7) expression (n = 5). (F) Dual-luciferase reporter assay of relative NCC marker genes (Foxd3, Snai2, Sox9, Pax7) influenced by β-catenin (n = 5). (G and H) Cdc42 pull-down activation assay in the shTrio and shMyh9 NCC groups, with quantification (n = 3). (I) Immunofluorescence staining of the Golgi body marker GM130 and F-actin for polarization assay. The nuclei were counterstained with DAPI. The following schematic shows a polarized and nonpolarized cell, as well as the angle of nucleus reorientation. Bar = 100 μm. The quantification is shown as the percentage of Golgi facing the wound and the angle of nucleus reorientation (n = 5). For (A), (E), (F), (H), and (I), data are represented as mean ± S.E.M. (two-tailed t test *p < 0.05, **p < 0.01).
Activation Assay Biochem Kit, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bk036+rac1/pm28707808-81-12-22?v=Cytoskeleton+Inc
Average 96 stars, based on 1 article reviews
activation assay biochem kit - by Bioz Stars, 2026-07
96/100 stars
  Buy from Supplier
pull down assay kits  (Cytoskeleton Inc)
96
Cytoskeleton Inc pull down assay kits
Myh9 assists Trio in the regulation of NCC migration via Rac1 and <t>Cdc42</t> GTPase activation. (A) Rac1 pull-down activation in the shCtrl, shTrio, and shMyh9 NCC groups, with quantification (n = 3). (B) Immunostaining of β-catenin (green) and Rac1 (red) in NCCs. Bar = 100 μm. (C) Immunostaining of β-catenin (green) and DAPI (blue) for nuclear β-catenin expression indicated by a white arrow in shTrio and shMyh9 NCCs. Bar = 100 μm. (D) Western blot of β-catenin in the nucleus and cytoplasm in the shTrio and shMyh9 NCC groups. (E) qRT-PCR of migratory NCC marker genes (Foxd3, Hnk-1, Snai2, Sox9, Twist-1, Pax7) expression (n = 5). (F) Dual-luciferase reporter assay of relative NCC marker genes (Foxd3, Snai2, Sox9, Pax7) influenced by β-catenin (n = 5). (G and H) Cdc42 pull-down activation assay in the shTrio and shMyh9 NCC groups, with quantification (n = 3). (I) Immunofluorescence staining of the Golgi body marker GM130 and F-actin for polarization assay. The nuclei were counterstained with DAPI. The following schematic shows a polarized and nonpolarized cell, as well as the angle of nucleus reorientation. Bar = 100 μm. The quantification is shown as the percentage of Golgi facing the wound and the angle of nucleus reorientation (n = 5). For (A), (E), (F), (H), and (I), data are represented as mean ± S.E.M. (two-tailed t test *p < 0.05, **p < 0.01).
Pull Down Assay Kits, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bk036+rac1/pmc05785464-121-12-15?v=Cytoskeleton+Inc
Average 96 stars, based on 1 article reviews
pull down assay kits - by Bioz Stars, 2026-07
96/100 stars
  Buy from Supplier

Image Search Results


Myh9 assists Trio in the regulation of NCC migration via Rac1 and Cdc42 GTPase activation. (A) Rac1 pull-down activation in the shCtrl, shTrio, and shMyh9 NCC groups, with quantification (n = 3). (B) Immunostaining of β-catenin (green) and Rac1 (red) in NCCs. Bar = 100 μm. (C) Immunostaining of β-catenin (green) and DAPI (blue) for nuclear β-catenin expression indicated by a white arrow in shTrio and shMyh9 NCCs. Bar = 100 μm. (D) Western blot of β-catenin in the nucleus and cytoplasm in the shTrio and shMyh9 NCC groups. (E) qRT-PCR of migratory NCC marker genes (Foxd3, Hnk-1, Snai2, Sox9, Twist-1, Pax7) expression (n = 5). (F) Dual-luciferase reporter assay of relative NCC marker genes (Foxd3, Snai2, Sox9, Pax7) influenced by β-catenin (n = 5). (G and H) Cdc42 pull-down activation assay in the shTrio and shMyh9 NCC groups, with quantification (n = 3). (I) Immunofluorescence staining of the Golgi body marker GM130 and F-actin for polarization assay. The nuclei were counterstained with DAPI. The following schematic shows a polarized and nonpolarized cell, as well as the angle of nucleus reorientation. Bar = 100 μm. The quantification is shown as the percentage of Golgi facing the wound and the angle of nucleus reorientation (n = 5). For (A), (E), (F), (H), and (I), data are represented as mean ± S.E.M. (two-tailed t test *p < 0.05, **p < 0.01).

Journal: Theranostics

Article Title: Trio cooperates with Myh9 to regulate neural crest-derived craniofacial development

doi: 10.7150/thno.51745

Figure Lengend Snippet: Myh9 assists Trio in the regulation of NCC migration via Rac1 and Cdc42 GTPase activation. (A) Rac1 pull-down activation in the shCtrl, shTrio, and shMyh9 NCC groups, with quantification (n = 3). (B) Immunostaining of β-catenin (green) and Rac1 (red) in NCCs. Bar = 100 μm. (C) Immunostaining of β-catenin (green) and DAPI (blue) for nuclear β-catenin expression indicated by a white arrow in shTrio and shMyh9 NCCs. Bar = 100 μm. (D) Western blot of β-catenin in the nucleus and cytoplasm in the shTrio and shMyh9 NCC groups. (E) qRT-PCR of migratory NCC marker genes (Foxd3, Hnk-1, Snai2, Sox9, Twist-1, Pax7) expression (n = 5). (F) Dual-luciferase reporter assay of relative NCC marker genes (Foxd3, Snai2, Sox9, Pax7) influenced by β-catenin (n = 5). (G and H) Cdc42 pull-down activation assay in the shTrio and shMyh9 NCC groups, with quantification (n = 3). (I) Immunofluorescence staining of the Golgi body marker GM130 and F-actin for polarization assay. The nuclei were counterstained with DAPI. The following schematic shows a polarized and nonpolarized cell, as well as the angle of nucleus reorientation. Bar = 100 μm. The quantification is shown as the percentage of Golgi facing the wound and the angle of nucleus reorientation (n = 5). For (A), (E), (F), (H), and (I), data are represented as mean ± S.E.M. (two-tailed t test *p < 0.05, **p < 0.01).

Article Snippet: The activities of Rac1 and Cdc42 were measured using Rac1 and Cdc42 pull-down Activation Assay Biochem Kit (BK035/BK036, Cytoskeleton Inc) according to the manufacturer's instructions.

Techniques: Migration, Activation Assay, Immunostaining, Expressing, Western Blot, Quantitative RT-PCR, Marker, Luciferase, Reporter Assay, Immunofluorescence, Staining, Two Tailed Test

myh9 and ca-Rac1/ca-Cdc42 partly restore the defects in trio morphants. (A) Bright field image of zebrafish embryos at 96 hpf. Bar = 100 μm. Red dotted line: mandible. “+”, coinjection. MO, morpholino. hpf, hours post-fertilization. c : con MO, t : trio MO, m : myh9 MO, t+m : trio MO+ myh9 mRNA, t+R : trio MO+Rac1 mRNA, t+C : trio MO+Cdc42 mRNA, m+R : myh9 MO+Rac1 mRNA, m+C : myh9 MO+Cdc42 mRNA. (B, C) Images and quantification of Alcian blue-stained zebrafish embryos at 120 hpf (n = 10). Bar = 500 μm. (D) Eye iridophore amount and distribution of the experimental groups (n = 10). Bar = 100 μm. (E) Time-lapse images of live Tg( sox10:egfp ) zebrafish at 14 and 16 SS in the experimental groups mentioned above. Bar = 100 μm. SS, somite stage. For (B) and (D), data are represented as mean ± S.E.M. (two-tailed t test *p < 0.05, **p < 0.01).

Journal: Theranostics

Article Title: Trio cooperates with Myh9 to regulate neural crest-derived craniofacial development

doi: 10.7150/thno.51745

Figure Lengend Snippet: myh9 and ca-Rac1/ca-Cdc42 partly restore the defects in trio morphants. (A) Bright field image of zebrafish embryos at 96 hpf. Bar = 100 μm. Red dotted line: mandible. “+”, coinjection. MO, morpholino. hpf, hours post-fertilization. c : con MO, t : trio MO, m : myh9 MO, t+m : trio MO+ myh9 mRNA, t+R : trio MO+Rac1 mRNA, t+C : trio MO+Cdc42 mRNA, m+R : myh9 MO+Rac1 mRNA, m+C : myh9 MO+Cdc42 mRNA. (B, C) Images and quantification of Alcian blue-stained zebrafish embryos at 120 hpf (n = 10). Bar = 500 μm. (D) Eye iridophore amount and distribution of the experimental groups (n = 10). Bar = 100 μm. (E) Time-lapse images of live Tg( sox10:egfp ) zebrafish at 14 and 16 SS in the experimental groups mentioned above. Bar = 100 μm. SS, somite stage. For (B) and (D), data are represented as mean ± S.E.M. (two-tailed t test *p < 0.05, **p < 0.01).

Article Snippet: The activities of Rac1 and Cdc42 were measured using Rac1 and Cdc42 pull-down Activation Assay Biochem Kit (BK035/BK036, Cytoskeleton Inc) according to the manufacturer's instructions.

Techniques: Staining, Two Tailed Test